Effects of ATP in perfused rat hind-limb preparation
نویسندگان
چکیده
منابع مشابه
Autologous pump-perfused rat hind limb preparation for investigating muscle function and metabolism in vivo.
OBJECTIVE Oxygen delivery, underpinned by vascular tone, is the principle limiting factor in the study of skeletal muscle physiology, particularly during muscle contraction. The aim of this study was to develop an autologous perfused rat hind limb preparation for the study of skeletal muscle contractile function. METHODS Adult Wistar rats were surgically prepared using a by-pass system for pu...
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A new protocol has been developed for selective removal of the endothelium from the arteries of the perfused rat hind-limb preparation. The hind limb was perfused with oxygenated Krebs-Ringer bicarbonate solution with a combination of high flow and timed air bubbles (2 minutes at high flow followed by 5 minutes of air bubbles, followed by a further 2 minutes at high flow). Representative vessel...
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OBJECTIVES Methods are needed for quantifying muscle deconditioning due to immobilization, aging, or spaceflight. Electrical impedance myography (EIM) is one technique that may offer easy-to-follow metrics. Here, we evaluate the time course and character of the change in single- and multi-frequency EIM parameters in the hind-limb suspension model of muscle deconditioning in rats. METHODS Sixt...
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We developed a novel method to stimulate the sympathetic innervation of the isolated, perfused rat hind limb to investigate whether a subpressor concentration of angiotensin II (Ang II) facilitates noradrenergic transmission in the vascular bed to skeletal muscle. We electrically stimulated the lumbar sympathetic trunk while perfusing the preparation with artificial medium. Seventy-five percent...
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The technic for isolation-perfusion of the rat hind limb is described. By the stand ardized technic, limb viability was well maintained, and leakage of anticancer agents into the systemic circulation was negligible. The gross and microscopic response of the Walker 256 carcinosarcoma to perfusion with triethylenethiophosphoramide is de scribed. The preparation permits study of the many variables...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1999
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)34678-5